7Z4L PDB NFT

SpCas9 bound to 18-nucleotide complementary DNA substrate in the checkpoint state

MOLNFT AF v1 smart contract in GenesisL1 blockchain:

0xDE3723766Bc32dcACD03C17BaA400A7B36837Eba

IPFS of NFT metadata:

bafybeieoapoqe5kni2pl7dmiiq424ymlxck2cw5waotz4hpyi4nf7c4irq/metadata.json

IPFS of structure file:

bafybeiaibmferj4w5dmhqyrutofrx5u4e6tbubynopw37d6fo7pmksbxtu/7z4l.pdb

MOLNFT PDB v1

NFT id:
190887
PDB ID:
7Z4L
Name:
SpCas9 bound to 18-nucleotide complementary DNA substrate in the checkpoint state
Keywords:
HYDROLASE
Experimental Method:
ELECTRON MICROSCOPY
Resolution:
0.00

NFT DeSci data

MolNFT PDB v1 is The Protein Data Bank database (URL: https://rcsb.org) of protein structures minted as ERC-721 NFT tokens on GenesisL1 blockchain for public free use, research and entertainment:

H.M. Berman, J. Westbrook, Z. Feng, G. Gilliland, T.N. Bhat, H. Weissig, I.N. Shindyalov, P.E. Bourne.
(2000) The Protein Data Bank Nucleic Acids Research, 28: 235-242.

MOLNFT 7Z4L research and analysis (ICN3D with VR)

FASTA sequences

>7z4l_A mol:na length:102 sgRNA
GGGACGCAUAAAGAUGAGACGCGUUUUAGAGCUAGAAAUAGCAAGUUAAAAUAAGGCUAGUCCGUUAUCAACUUGAAAAAGUGGCACCGAGUCGGUGCUUUU
>7z4l_B mol:protein length:1368 CRISPR-associated endonuclease Cas9/Csn1
MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKNLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESFLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKFRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQIGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLRKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGNSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYFTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDSVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERLKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRHKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYLYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPSEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHVAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLNAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTEITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGITIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNELALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLDATLIHQSITGLYETRIDLSQLGGD
>7z4l_C mol:na length:38 Target strand of 18-nucleotide complementary DNA substrate
CGTGATTCCAGCGTCTCATCTTTATGCGAGTGTACTCG
>7z4l_D mol:na length:38 Non-target strand of 18-nucleotide complementary DNA substrate
CGAGTACACTATTGCGGGACGCTATTATTGGAATCACG

References and links

Current molecular structure file and data about current molecular structure file and NFT token based on it with
PDBID: 7Z4L
TITLE: SpCas9 bound to 18-nucleotide complementary DNA substrate in the checkpoint state
DOI:
AUTHORS: Pacesa, M., Jinek, M.
ACCESSION DATE: 03/04/22
Obtained at RCSB PDB https://rcsb.org
RCSB Link: https://www.rcsb.org/structure/7Z4L