7Z4J PDB NFT

SpCas9 bound to 18-nucleotide complementary DNA substrate in the catalytic state

MOLNFT AF v1 smart contract in GenesisL1 blockchain:

0xDE3723766Bc32dcACD03C17BaA400A7B36837Eba

IPFS of NFT metadata:

bafybeicvdrnn5j3ojsz7sbko2co4sgljlum2qs43qre5oihnzzd4wqhj6u/metadata.json

IPFS of structure file:

bafybeigzc67qtyzge7oxcx3hcf4dvy4xx2mweabdcxg3xrldd4rkmc4apq/7z4j.pdb

MOLNFT PDB v1

NFT id:
190885
PDB ID:
7Z4J
Name:
SpCas9 bound to 18-nucleotide complementary DNA substrate in the catalytic state
Keywords:
HYDROLASE
Experimental Method:
ELECTRON MICROSCOPY
Resolution:
2.99

NFT DeSci data

MolNFT PDB v1 is The Protein Data Bank database (URL: https://rcsb.org) of protein structures minted as ERC-721 NFT tokens on GenesisL1 blockchain for public free use, research and entertainment:

H.M. Berman, J. Westbrook, Z. Feng, G. Gilliland, T.N. Bhat, H. Weissig, I.N. Shindyalov, P.E. Bourne.
(2000) The Protein Data Bank Nucleic Acids Research, 28: 235-242.

MOLNFT 7Z4J research and analysis (ICN3D with VR)

FASTA sequences

>7z4j_A mol:na length:102 sgRNA
GGGACGCAUAAAGAUGAGACGCGUUUUAGAGCUAGAAAUAGCAAGUUAAAAUAAGGCUAGUCCGUUAUCAACUUGAAAAAGUGGCACCGAGUCGGUGCUUUU
>7z4j_c mol:na length:13 Target strand of 18-nucleotide complementary DNA substrate, PAM-proximal end
CGTGATTCCAGCG
>7z4j_C mol:na length:32 Target strand of 18-nucleotide complementary DNA substrate, PAM-distal end
GTCTCATCTTTATGCGAGTGTACTCGTCTTGC
>7z4j_D mol:na length:44 Non-target strand of 18-nucleotide complementary DNA substrate
GCAAGACGAGTACACTCGCATAAAACGCTAATATTGGAATCACG
>7z4j_B mol:protein length:1368 CRISPR-associated endonuclease Cas9/Csn1
MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKNLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESFLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKFRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQIGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLRKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGNSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYFTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDSVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERLKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRHKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYLYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPSEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHVAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLNAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTEITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGITIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNELALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLDATLIHQSITGLYETRIDLSQLGGD

References and links

Current molecular structure file and data about current molecular structure file and NFT token based on it with
PDBID: 7Z4J
TITLE: SpCas9 bound to 18-nucleotide complementary DNA substrate in the catalytic state
DOI:
AUTHORS: Pacesa, M., Jinek, M.
ACCESSION DATE: 03/03/22
Obtained at RCSB PDB https://rcsb.org
RCSB Link: https://www.rcsb.org/structure/7Z4J