7Z4E PDB NFT

SpCas9 bound to 8-nucleotide complementary DNA substrate

MOLNFT AF v1 smart contract in GenesisL1 blockchain:

0xDE3723766Bc32dcACD03C17BaA400A7B36837Eba

IPFS of NFT metadata:

bafybeifx66s37hglshmtzzs6lc6abkbqi7fmerejddoczu4dbqfdujv34i/metadata.json

IPFS of structure file:

bafybeiepflucw5qxc7ujcak67l7pl736azuo2ibl3tlmlks3ozmqf6spfe/7z4e.pdb

MOLNFT PDB v1

NFT id:
190880
PDB ID:
7Z4E
Name:
SpCas9 bound to 8-nucleotide complementary DNA substrate
Keywords:
HYDROLASE
Experimental Method:
ELECTRON MICROSCOPY
Resolution:
4.14

NFT DeSci data

MolNFT PDB v1 is The Protein Data Bank database (URL: https://rcsb.org) of protein structures minted as ERC-721 NFT tokens on GenesisL1 blockchain for public free use, research and entertainment:

H.M. Berman, J. Westbrook, Z. Feng, G. Gilliland, T.N. Bhat, H. Weissig, I.N. Shindyalov, P.E. Bourne.
(2000) The Protein Data Bank Nucleic Acids Research, 28: 235-242.

MOLNFT 7Z4E research and analysis (ICN3D with VR)

FASTA sequences

>7z4e_B mol:protein length:1368 CRISPR-associated endonuclease Cas9/Csn1
MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKNLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESFLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKFRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQIGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLRKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGNSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYFTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDSVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERLKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRHKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYLYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPSEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHVAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLNAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTEITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGITIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNELALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLDATLIHQSITGLYETRIDLSQLGGD
>7z4e_A mol:na length:102 sgRNA
GGGACGCAUAAAGAUGAGACGCGUUUUAGAGCUAGAAAUAGCAAGUUAAAAUAAGGCUAGUCCGUUAUCAACUUGAAAAAGUGGCACCGAGUCGGUGCUUUU
>7z4e_C mol:na length:30 Target strand of 8 nucleotide complementary DNA substrate
CGTGATTCCAGCGTCTCAAAGTCTACATAG
>7z4e_D mol:na length:30 Non-target strand of 8 nucleotide complementary DNA substrate
CTATGTAGACTTACTATTATTGGAATCACG

References and links

Current molecular structure file and data about current molecular structure file and NFT token based on it with
PDBID: 7Z4E
TITLE: SpCas9 bound to 8-nucleotide complementary DNA substrate
DOI:
AUTHORS: Pacesa, M., Jinek, M.
ACCESSION DATE: 03/03/22
Obtained at RCSB PDB https://rcsb.org
RCSB Link: https://www.rcsb.org/structure/7Z4E